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URN etd-0904113-162221 Statistics This thesis had been viewed 3022 times. Download 3495 times. Author Da-Wei Chang Author's Email Address No Public. Department Bioengineering Year 2012 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 64 Title Effect of aeration on the growth of lactic acid bacteria Keyword Lactic acid bacteria Oxidation-reduction potenti Lactic acid bacteria Oxidation-reduction potenti Abstract In this study, Lactobacillus casei Shirota from Yakult and Lacto-bacillus acidophilus from AB yogurt of the President Enterprise Corporation were selected as the tested bacteria to study the aeration on the growth of lactic acid bacteria.
The two Lactobacillus strains were cultured under aeration, micro-aeration, and anaerobic conditions in a 5L fermenters using MRS medium at 37?C, pH 5. The cell concentrations, ORP, production of lactic acid and consumption of glucose were measured in the three aeration conditions of the fermentation. Effect of cell growth for the two Lactobacillus on aeration and ORP was discussed in this study.
The results indicated that the maximum cell concentration and lactic acid production were OD600nm 20.55±0.21 and 31.4 g/L respectively for Lactobacillus casei Shirota under anaerobic conditions, and those for Lactobacillus acidophillus were OD600nm 11.95±0.17 and 24.6g/L.
In this study, ORP was very close related to cell growth of the two Lactobacillus. ORP decreased continuously during the exponential growth phase under anaerobic or micro-aerobic conditions. When ORP remained constant or increased, cell growth was in stationary phase or cell lysis was observed. It was interesting to note that increasing cell lysis was observed under micro-aeration when the measured ORP increased.
Advisor Committee Kow-Jen Duan - advisor
Tsong-Rong Yan - co-chair
Wen-Chien Lee - co-chair
Files Date of Defense 2013-07-29 Date of Submission 2013-09-05