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Title page for etd-0906104-164749


URN etd-0906104-164749 Statistics This thesis had been viewed 2108 times. Download 22 times.
Author Chi-Fu Chou
Author's Email Address g9109011@ttu.edu.tw
Department Bioengineering
Year 2003 Semester 2
Degree Master Type of Document Master's Thesis
Language English Page Count 95
Title STRUCTRURE GENE ANALYSIS OF
ASPERGILLUS NIGER β-GLUCOSIDASE II
Keyword
  • transglycosylation
  • sequence
  • regularion
  • glucosidase
  • aspergillus niger
  • aspergillus niger
  • glucosidase
  • regularion
  • sequence
  • transglycosylation
  • Abstract β-Glucosidase is a member of glycosidase family that catalyzes the transfer of glycosyl group between oxygen nucleophiles. Because of its retaining mechanism, transglycosylation may occurs when the retained glucose released by aglycone other than water. In the previous study, β-glucosidase II was isolated from Aspergillus niger. Besides the typical hydrolysis activity, it was found that processes transglycosylation activity toward specific substrates. Cello-oligosaccharide was synthesized from cellobiose, and alkyl-glucosides were synthesized when supplied with alcohol. The products may apply to functional food and pharmaceutical industrial. For the purpose of enzyme cloning, properties enhancement, and regulation comprehension; we designed primers from known b-glucosidase sequences and succeed in obtaining the DNA and RNA-reversed-transcribed cDNA sequence of A. niger β-glucosidase II. The cloned genomic sequence revealed a 2,948 bp open reading frame with 6 introns and 7 exons. The cDNA showed a 2,583 sequence encoding an 860-amono-acid protein that belongs to glycosyl hydrolase family 3 as well as identified as a member of β-glucosidase subfamily B.
    Advisor Committee
  • Tsong-Rong Yan - advisor
  • Chorng-Liang Pan - co-chair
  • Ming-Tse Lin - co-chair
  • Files indicate in-campus access at one year and off-campus not accessible
    Date of Defense 2004-07-28 Date of Submission 2004-09-06


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