||In this study, basswoods of Cinnamomum sp. including Cinnamomum micranthum, Cinnamomum camphora and Cinnamomum kanehirae Hayata located at northern, central, southern and eastern Taiwan were collected. Ultrasonic-assisted extraction of samples with ethanol was performed at different temperature, followed by thin layer chromatography (TLC) with three mobile phases, respectively. Thereafter, the differentiation between these chromatograms was evaluated. The results showed that the extraction yields of the test samples increased with increasing temperature. The highest yield was found when sample was extracted at 80℃ for 20 minutes. Meanwhile, extraction temperature rather than extraction time affected the chromatogram pattern of tested samples subjected to TLC followed by UV irradiation of 365nm. The chromatograms exhibited significant difference when extraction temperature was 60℃.In addition, the chromatograms of Cinnamomum sp. also showed significantly different when 100% chloroform (C), toluene: ethyl acetate (8: 2) (E) and toluene: acetone (8: 2) (A)were used as the mobile phases, respectively. Intense fluorescence spots of Rf value of 0, labeled as C1, E1 and A1, respectively, were noted in the chromatograms of C. micranthum. In addition, one or two more spots were also found when compared with chromatograms of C.camphora and C.kanehirae. More spots, i. e. Rf value of 0.867 (labeled as C2), Rf value of 0.911 (labeled as C3), Rf value of 0.467 (labeled as E3)and Rf value of 0.511 (labeled as A3)in the chromatograms of C.camphorawere obtained, which did not found in those of C.micranthum and C.kanehirae. Based on the difference of the chromatograms of TLC,the differentiation between C.micranthum, C.camphora and C.kanehirae might be preliminarily determined.