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URN etd-0908106-160841 Statistics This thesis had been viewed 2737 times. Download 1645 times. Author Yu-Chun Hsueh Author's Email Address firstname.lastname@example.org Department Bioengineering Year 2005 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 160 Title QUANTIFICATION OF DENITRIFYING BACTERIA IN SLUDGE FOR THE TREATMENT OF WASTEWATER WITH NITRATE BY QUANTITATIVE PCR Keyword Denitrification Quantitative PCR Denitrifying bacteria Denitrifying bacteria Quantitative PCR Denitrification Abstract The nitrogen cycle contains autotrophic nitrification and heterotrophic denitrification. The bacteria population and the chemical reaction were very complex to dispose the waste water with nitrate. We must to setup an on-line detectable system to quantify the bacteria population in the nitrate waste water for the evaluation of waste water treatment.
The batch reaction (BR) and continue stirred tank reaction (CSTR) were setup to simulate the treatment of waste water with nitrate. Therefore, the molecular biologic assay was developed to detect the denitrifying bacteria on-line. There are many enzymes involving denitrification of nitrogen cycle, the nirK and nirS were selected to design the primer pairs for polymerase chain reaction. These primer pairs with the genomic DNAs of Alcaligenes xylosoxidans BCRC 12838 (nirK type), Pseudomonas fluorescens BCRC 16016 (nirS type), and denitrifying bacteria in wastewater carried out the polymerase chain reaction. The primer sets of nirK4f-5r and nirS2f-3r were specific to the respective standard denitrifying bacterial strains, so these two primer sets were selected for quantitative competitive polymerase chain reaction (QC-PCR) to rapidly quantify the two type of denitrifying bacteria in active sludge of denitrifying bioreactor.
The denitrification yield of simulated batch-type denitrifying bioreactor with 500 ppm nitrate were 28%, 57%, 86%, and 89% in 6 hr at 20?C, 25?C, 30?C, and 35?C, respectively. The copies of nirk and nirS in the bioreactors at 25?C and 30?C within 6 hr were obvious increase quantified by QC-PCR. However, treated at low temperature decreased the denitrifying bacteria in active sludge. It was evident that temperature effects the growth of denitrifying bacteria. In continuous stirred tank with HRT 100ml/hr, the denitrification yield was up to 90% and maintained over 80% under the continuous operation for 150 hr. The temperature was also effect the growth of denitrifying bacteria that elevated at 30 ?C and suppressed at 20 ?C.
Advisor Committee Ming-Tse Lin - advisor
Chi-Tsai Lin - co-chair
Chien-Hsien Chen - co-chair
Files Date of Defense 2006-06-09 Date of Submission 2006-09-08