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URN etd-0909115-115810 Statistics This thesis had been viewed 1573 times. Download 94 times. Author Yi-hsuan Chen Author's Email Address No Public. Department Bioengineering Year 2014 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 93 Title Studies on micropropagation and secondary metabolites content of several strawberry (Fragaria × ananassa) cultivars Keyword strawberry secondary metabolites micropropagation micropropagation secondary metabolites strawberry Abstract For germination rate test, fourteen sources of strawberry seeds were cultured in sterile water or on Murashiage and Skoog (1962) medium without plant growth regulator after surface sterilization. The results showed that seeds soaked in sterile water have higher germination rate than those cultured on medium. However, the rate of germination was from 17.54 to 98.63 %, which depends on different sources of seeds. The runner or shoots from Taoyuan No.4, Perfume, Toyonoka and Large Toyonoka were used as explants, which cultured on MS basal salts containing different concentrations of antioxidants, Polyvinylpyrrolidone (PVP), and added individually or in different combinations with growth regulators for shoot multiplication. The best response was the Toyonoka’s runner shoots, multiple shoots were formed when cultured in 1/2MS basal medium supplemented with 5 mg/l PVP ,1.0 mg/l Thidiazuron (TDZ) and 0.5 mg/l Indole-3-butyric acid (IBA) after four months. Callus and adventitious shoots were regenerated from the edge of Toyonoka leaf explants cultured on 1/2MS basal medium supplemented with 5 mg/l PVP, 1 mg/l TDZ and 0.1 mg/l 2,4-Dichlorophenoxyacetic acid (2,4-D) after 2 weeks.
Methanol extracts from fourteen samples of strawberry were analyzed. The shoots and leaves from field-grown Toyonoka’s plant expressed highest content of total flavonoids (20.256 eq. quercetin μg/mg DW) and total phenols (130.298 eq. gallic acid μg/mg DW),and the amount of catechin, epicatechin, procyanidin B1 and procyanidin B2 were 20.739μg/mg DW, 2.967 μg/mg DW, 4.953 μg/mg DW and 8.563 μg/mg DW, respectively.
Advisor Committee Chin-wen Ho - advisor
none - co-chair
none - co-chair
Files Date of Defense 2015-07-30 Date of Submission 2015-09-09