| Abstract |
We collected the 1,281 buccal swab samples for typing Y chromosome DNA and identifying paternity relationship with written informed consent from unrelated male volunteers of 14 ethnic groups residing in Taiwan. The donators included Holo, Hakka, Mainlander, Amis, Paiwan, Atayal, Bunun, Truku, Rukai, Puyuma, Tsou, Saisiyat, Yami and Thao. We got 923 DNA Y-STR haplotypes for identifying paternal affinity.
Allele frequencies for the 17 Y-specific short tandem repeats (Y-STRs) loci DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 and Y-GATA-H4 (AmpFℓSTR® Yfiler™ PCR Amplification Kit) were determined from 1,281 samples. The genetic distance values below 0.2, which show high genetic affinity between compared groups, have four groups: (1) Holo, Hakka and Mainlander; (2) Paiwan and Rukai; (3) Paiwan and Puyuma; (4) Atayal and Truku.
From the relation of genetic blood and geographic environment, we found the Atayal, Bunun, Truku and Tsou tribes had the high frequencies over 78.6 % of allele 13 and the Amis, Paiwan, Rukai, Puyuma, Saisiyat, Yami and Thao tribes had the high frequencies over 76.1 % of allele 12.
In 60 paternity cases, we found 4 cases happening mutations on DNA STR and used DNA Y-STR to determine the blood relation for these. |