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Title page for etd-0911107-114858


URN etd-0911107-114858 Statistics This thesis had been viewed 2815 times. Download 21 times.
Author Shun-Jen Cheng
Author's Email Address No Public.
Department Bioengineering
Year 2006 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 66
Title Antioxidant Capacity Guided Fractionation from
Methanol Extract of Galla Rhois
Keyword
  • reducing power
  • DPPH
  • chelating
  • Galla Rhois
  • antioxidant
  • Rhus chinensis Mill
  • Rhus chinensis Mill
  • antioxidant
  • Galla Rhois
  • chelating
  • DPPH
  • reducing power
  • Abstract Galla rhois, the dry gall on the leaves of Rhus chinensis
    (Anacardiaceae), was used in traditional Chinese medicine. It exhibited
    some pharmacological properties, including anti-ulcer, anti-clotting,
    antibacterial and repression of prostaglandin synthesis. The effects of
    solvent varieties on the antioxidant activity of Gall rhois and antioxidant
    capacity-guided fractionation were conducted in this study. The results
    showed that the extraction yield, ca. 87.16 %, was the highest when 80%
    ethanol was used as extraction solvent. DPPH free radical scavenging
    activity of 80% ethanol extract was higher than those of methanol extract
    and hot water extract. The methanol extract showed demonstrated the
    highest reducing power and ferrous ion chelating activity. The efficient
    concentration of chelating 50% of ferrous ions (EC50) was 2.599 mg/ ml. The
    highest content of total phenolic, ca. 797.0 mg gallic acid / g, was found in
    hot water extract. Methanol extract was partitioned sequentially with
    n-hexane, diethyl ether, ethyl acetate, 1-butanol and water and then the
    antioxidant activity of each fraction was determined. The DPPH free
    radical scavenging activity and reducing power were highest in ethyl
    III
    acetate fraction. On the other hand, the water fraction exhibited the
    highest ferrous ion chelating activity.
    The highest phenolic content, expressed as 504, 8 mg of gallic acid per
    grem of fraction, was obtained in ethyl acetate fraction.
    Advisor Committee
  • Shiow-Ling Lee - advisor
  • none - co-chair
  • none - co-chair
  • Files indicate in-campus access only
    Date of Defense 2007-07-31 Date of Submission 2007-09-11


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