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Title page for etd-0912117-115310


URN etd-0912117-115310 Statistics This thesis had been viewed 256 times. Download 0 times.
Author Chun-ling Chen
Author's Email Address No Public.
Department Bioengineering
Year 2016 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 55
Title Microbial biodiesel production by acid-catalyzed direct transesterification of Rhodotorula glutinis biomass
Keyword
  • acid-catalyzed
  • direct transesterification
  • microbial biodiesel
  • Rhodotorula glutinis
  • Rhodotorula glutinis
  • microbial biodiesel
  • direct transesterification
  • acid-catalyzed
  • Abstract Biodiesel, one of the most prominent renewable energy, is conventionally produced from vegetable oils or animal fats by transesterification of triacylglycerols with methanol or ethanol to yield the corresponding fatty acid monoalkyl esters. However, it presents some drawbacks including the high manufacturing cost, the competition with food supply and potential environmental impacts. In this study, the oleaginous yeast, Rhodotorula glutinis was used for the direct transesterification without lipid pre-extraction to produce biodiesel. Because free fatty acids are usually present in the lipid composition, sulfuric acid was chosen as a catalyst for the transesterification reactions with methanol to avoid soap formation from free fatty acid neutralization. Crude glycerol (the byproduct from biodiesel production) was used as a sole carbon source for R. glutinis cultivation, giving rise to the lipid concentration up to 1.2 g/l. Under the same culture condition using glucose or pure glycerol as a sole carbon source instead, the lipid concentrations obtained were only 0.6 and 0.5 g/l, respectively. The effects of catalyst concentration, reaction temperature, reaction time and methanol loading on the transesterification level were also examined. When 1 g of R. glutinis dried biomass to methanol ratio of 1:20 (w/v) was subject to direct transesterification catalyzed by 0.6 M H2SO4 at 70oC for 20 h, the fatty acid methyl ester yield reached 111%.
    Advisor Committee
  • I-ching Kuan - advisor
  • Chi-yang Yu - co-chair
  • Hong-wei Yen - co-chair
  • Files indicate in-campus access at 5 years and off-campus access at 5 years
    Date of Defense 2017-07-21 Date of Submission 2017-09-12


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