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Title page for etd-0912118-122331


URN etd-0912118-122331 Statistics This thesis had been viewed 80 times. Download 0 times.
Author Wen-Ann Huang
Author's Email Address No Public.
Department Bioengineering
Year 2017 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 72
Title Tissue culture and evaluation of antioxidant activity of Loropetalum chinensis (R.Br) Oliv
Keyword
  • Loropetalum chinensis (R.Br) Oliv
  • Loropetalum chinensis (R.Br) Oliv
  • Abstract This study was established a method for the shoot proliferation and mass production of callus of Loropetalum chinensis (R. Br. Oliv.). Stem segment with 2 nodes (nodal explant) were cultured on C2D basal medium supplemented with 1.0 mg l-1 gibberellic acid (GA3) and 150 ml l-1 coconut water (CW) for 4 weeks showed the highest shoot sprouting rate (93 %) and the average number of shoots (1.6 ± 0.47). The best percentage of shoot elongation was 93 % when the explant cultured on WPM basal medium contained with 1.0 mg l-1 GA3 and 150 ml l-1 CW. The regeneration rate of adventitious root was 26.7% when adventitious shoots cultured on 1/2 MS basal medium contained with (2 or 3 mg l-1) IBA. The proliferation rate of callus was 5.38-fold when callus cultured on MS basal medium supplemented with 0.5 mg l-1 1-naphthaleneacetic acid (NAA)、0.5 mg l-1 6-benzylaminopurine (BA) and 1.0 mg l-1 thidiazuron (TDZ) for 6 weeks.
    Callus from different cultural media and extracted were 75 % ethanol at room temperature. The total phenolic content of extracts were between 50.77 ± 2.44 ~ 81.09 ± 4.45 mg gallic acid eq./g DW. The total flavonoid content was between 1.96 ± 0.03~5.41 ± 0.05 mg quercetin eq./g D.W. Gallic acid and myricetin were showed in methanol extracts of callus by thin layer chromatography(TLC) analysis.
    Advisor Committee
  • Chin-Wen Ho - advisor
  • Chien-Hsien Chen - co-chair
  • Ji-Wei Huang - co-chair
  • Files indicate in-campus access at 2 years and off-campus access at 2 years
    Date of Defense 2018-07-30 Date of Submission 2018-09-12


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