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The defense date of the thesis is 2018-09-12
The current date is 2019-05-19
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URN etd-0912118-122331 Statistics This thesis had been viewed 97 times. Download 0 times. Author Wen-Ann Huang Author's Email Address No Public. Department Bioengineering Year 2017 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 72 Title Tissue culture and evaluation of antioxidant activity of Loropetalum chinensis (R.Br) Oliv Keyword Loropetalum chinensis (R.Br) Oliv Loropetalum chinensis (R.Br) Oliv Abstract This study was established a method for the shoot proliferation and mass production of callus of Loropetalum chinensis (R. Br. Oliv.). Stem segment with 2 nodes (nodal explant) were cultured on C2D basal medium supplemented with 1.0 mg l-1 gibberellic acid (GA3) and 150 ml l-1 coconut water (CW) for 4 weeks showed the highest shoot sprouting rate (93 %) and the average number of shoots (1.6 ± 0.47). The best percentage of shoot elongation was 93 % when the explant cultured on WPM basal medium contained with 1.0 mg l-1 GA3 and 150 ml l-1 CW. The regeneration rate of adventitious root was 26.7% when adventitious shoots cultured on 1/2 MS basal medium contained with (2 or 3 mg l-1) IBA. The proliferation rate of callus was 5.38-fold when callus cultured on MS basal medium supplemented with 0.5 mg l-1 1-naphthaleneacetic acid (NAA)、0.5 mg l-1 6-benzylaminopurine (BA) and 1.0 mg l-1 thidiazuron (TDZ) for 6 weeks.
Callus from different cultural media and extracted were 75 % ethanol at room temperature. The total phenolic content of extracts were between 50.77 ± 2.44 ~ 81.09 ± 4.45 mg gallic acid eq./g DW. The total flavonoid content was between 1.96 ± 0.03~5.41 ± 0.05 mg quercetin eq./g D.W. Gallic acid and myricetin were showed in methanol extracts of callus by thin layer chromatography(TLC) analysis.
Advisor Committee Chin-Wen Ho - advisor
Chien-Hsien Chen - co-chair
Ji-Wei Huang - co-chair
Files Date of Defense 2018-07-30 Date of Submission 2018-09-12