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Title page for etd-0913117-145837


URN etd-0913117-145837 Statistics This thesis had been viewed 238 times. Download 0 times.
Author Yun-Ru Huang
Author's Email Address No Public.
Department Bioengineering
Year 2016 Semester 2
Degree Master Type of Document Master's Thesis
Language zh-TW.Big5 Chinese Page Count 102
Title Develop a Cell-EIS biosensor for real-time monitoring cell growth
Keyword
  • Cell growth monitoring
  • Cell-EIS
  • TEER
  • Caco-2
  • Caco-2
  • TEER
  • Cell-EIS
  • Cell growth monitoring
  • Abstract There are many changes in the growth of cells, so we must interact with multiple parameters in order to truly establish a complete cell growth model. In this study, we will use the cell-quartz crystal microbalance system developed by our laboratory and combine the principles of Electrochemical impedance spectroscopy (EIS) and Trans-Epithelial Electric Resistance (TEER) A Cell-EIS real-time measurement system was used to monitor cell growth changes online. The results of the study with different solutions for Cell-EIS stability measurement, that the voltage and impedance performance showed a positive performance. Therefore, when measured by Cell-EIS, Caco-2 cells will gradually form a complete and dense cell monolayer because of the growth and differentiation process, thus affecting the conductivity, so the voltage (impedance) will change with The number of training days increased, and began to produce a higher measurement value. The results were compared using the results of the Cell-EIS system and found to be stable in the lateral measurement of cell density. During the stable growth period of Cac6-2 cells on days 11 and 16, the MTT assay was stable on day 11-14 The results of the same trend; the other to measure the integrity of the cross-cell membrane, the cell's stable growth period in the range of 9.83-16 days. And similar trends were observed with MTT assay and TEER as well as previous studies using Cell-QCM in this study. The growth of Caco-2 cells was significantly decreased after 24 hours of adding 10 μM of mitotonine on Caco-2 cells, which indicated that the density and integrity of cell monolayer had been harmed by drugs. The results of this study confirm that this system is a convenient and rapid measurement tool, can simultaneously measure the cell adhesion growth, differentiation process of cell monolayer membrane density and integrity changes. The results demonstrate that the Cell-EIS system can be used to monitor the changes in cell growth processes online and to be screened for anticancer drugs.
    Advisor Committee
  • Tsong-Rong Yan - advisor
  • none - co-chair
  • none - co-chair
  • Files indicate in-campus access at 2 years and off-campus access at 2 years
    Date of Defense 2017-07-26 Date of Submission 2017-09-13


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