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URN etd-0919107-114028 Statistics This thesis had been viewed 4158 times. Download 4337 times. Author Chien Pan Author's Email Address No Public. Department Bioengineering Year 2006 Semester 2 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 85 Title Determination of small biomolecule by fiber-optics surface plasma resonance sensor Keyword small biomolecule fiber-optics surface plasma resonance sensor fiber-optics surface plasma resonance sensor small biomolecule Abstract Fiber-optic surface plasmon resonance (SPR) can be used as a rapid and quantitative assay, and is also an important tool for monitoring biomolecular interactions. Furthermore, the use of fiber-optic SPR provides a number of potential advantages. Because the fiber optic sensors are reproducible and inexpensive to be disposable, binding kinetics and screening analyses can be performed in complex media without fear of irreversibly fouling expensive chipbased SPR platforms. Additionally, the small size of the fibers optic probe and sensing area make the sensors ideal for studying small volumes of samples. The applications are more and more than before.
In this study, we used the fiber-optic SPR sensor designed and obtained from Graduate Institute of Electro-Optical Engineering in Tatung University to determine the small biomolecules. The nanogold particle and precipitation of 4-choro-1-naphthol were used to amplify the detection signals of SPR. The amplification method using nanogold particle was insignificant, but one using precipitation by reducing 4-choro-1-naphthol was able to enhance significantly the SPR signals. The signal changes of wavelength shift were consistent to the increasing in the concentrations of biotin-conjugated albumin bovine serum (biotin-BSA) and ochratoxin A-conjugated ovalbumin (OTA-OVA). Therefore, these results indicated that the amplification method of reducing precipitation by enzyme was suitable to the fiber-optic SPR detection system. The detection limiting for biotin-BSA was 0~10 ?g/ml, and 10~20 ng/ml for biotin.
Advisor Committee Ming-Tse Lin - advisor
Chi-Hsien Liu - co-chair
Woo-Hu Tsai - co-chair
Files Date of Defense 2007-07-31 Date of Submission 2007-09-19