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URN etd-1130107-110202 Statistics This thesis had been viewed 4969 times. Download 8436 times. Author Ting-wei Huang Author's Email Address No Public. Department Bioengineering Year 2007 Semester 1 Degree Master Type of Document Master's Thesis Language zh-TW.Big5 Chinese Page Count 58 Title Andioxidant activity and content of isoflavones of ethanolic extract from callus of Cajanus cajan Keyword isoflavones isoflavones Abstract Three kinds of explants(cotyledon, hypocotyl and root) from Cajanus cajan were used to established callus culture. Ethanolic extract prepared from five plant materials, callus from cotyledon, callus from hypocotyl, callus from root, Cajanus cajan seeds and Glycine max seeds were examined for their phenolic contents, flavonoids contents and DPPH radical - scavenging activity. The content of isoflavone (genistein and daidzein) in the ethanolic extract was determined by high-performance liquid chromatography. The multiplication of callus was very fast and the biomass increase of callus from root reached to 13.8 ± 1.11 folds within 14 days culture. Among five plant materials, the ethanolic extract of root originated callus expressed high total phenolic contents (as gallic acid equivalents: 7.45 ± 0.14 mg /g D.W.) and high DPPH radical- scavenging activity (90 % at quarter strength of extract) . The content of isoflavone of callus only expressed genistein and the callus from cotyledon showed the highest total isoflavone contents which was 6 times of Glycine max seeds. Advisor Committee Chin-Wen Ho - advisor
Files Date of Defense 2007-11-13 Date of Submission 2008-01-13